Part:BBa_K133027:Design

HpaA-UreB-RGD-Histop

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 139
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 170
  Illegal BglII site found at 266
  Illegal BamHI site found at 1799
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1021
  Illegal AgeI site found at 2199
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2506
  Illegal SapI.rc site found at 907
  

Design Notes

The part was cloned into pSB1.AK3 without terminator for use in bacterial cells (surface expression).

Source

HpaA and LPP were ordered from GeneArt, so were primers for RGD-Histop. RGD-Histop was constructed with annealing.